Kristensen Michael, Jespersen Jørgen B
Danish Pest Infestation Laboratory, Skovbrynet 14, DK-2800 Kgs, Lyngby, Denmark.
J Econ Entomol. 2003 Aug;96(4):1300-6. doi: 10.1093/jee/96.4.1300.
We determined the toxicity of the two IGRs, diflubenzuron and cyromazine, in this survey of resistance in Danish field populations of Musca domestica (L.). We observed resistance toward diflubenzuron and for the first time in Denmark and we found field populations with some resistance to cyromazine. Eleven of the twenty-one field populations had larvae surviving a diagnostic dose of 1.6 times of susceptible LC95 of diflubenzuron and two of these populations had larvae surviving 6.1 times of LC95. Eight of the twenty-one field populations had larvae surviving 2.2 times of susceptible LC95 of cyromazine and one population had larvae surviving 4.4 times of LC95. A fivefold cyromazine resistant strain was established after selection with cyromazine. It was 3-, 5-, and 90-fold resistant to diflubenzuron, triflumuron, and methoprene, respectively. Two diflubenzuron resistant strains (120- and 86-fold, respectively) were established. They showed a high level of resistance to triflumuron (1000- and 200-fold, respectively), and moderate resistance to methoprene (73- and 50-fold, respectively). Both were susceptible to cyromazine. This study shows that by applying the recommendations of previous resistance risk assessments, severe control failures and detrimental development of a high level of resistance have been avoided. The development of resistance has not been completely avoided, but has not developed to a level of biological or economic importance.
在本次对丹麦家蝇（Musca domestica (L.)）田间种群抗性的调查中，我们测定了两种昆虫生长调节剂（IGRs）——除虫脲和灭蝇胺的毒性。我们观察到了对除虫脲的抗性，这在丹麦尚属首次，并且发现了对灭蝇胺具有一定抗性的田间种群。21个田间种群中有11个种群的幼虫在诊断剂量为敏感品系除虫脲LC95的1.6倍时存活，其中有2个种群的幼虫在LC95的6.1倍剂量下存活。21个田间种群中有8个种群的幼虫在诊断剂量为敏感品系灭蝇胺LC95的2.2倍时存活，有1个种群的幼虫在LC95的4.4倍剂量下存活。用灭蝇胺进行选择后建立了一个对灭蝇胺具有5倍抗性的品系。该品系对除虫脲、氟铃脲和烯虫酯的抗性分别为3倍、5倍和90倍。建立了两个对除虫脲具有抗性的品系（分别为120倍和86倍）。它们对氟铃脲表现出高水平抗性（分别为1000倍和200倍），对烯虫酯表现出中等抗性（分别为73倍和50倍）。二者对灭蝇胺均敏感。本研究表明，通过应用先前抗性风险评估的建议，避免了严重的防治失败以及高水平抗性的有害发展。抗性的发展并未完全避免，但尚未发展到具有生物学或经济重要性的水平。
