Heyer A, Gatz C
Institut für Genbiologische Forschung Berlin GmbH, Germany.
Plant Mol Biol. 1992 Nov;20(4):589-600. doi: 10.1007/BF00046444.
We have isolated and sequenced overlapping genomic and cDNA clones encoding the apoprotein of a potato phytochrome. Based on the deduced amino acid sequence, which shows 77% identity to the Arabidopsis phyB and 50% identity to the potato phyA open reading frame, we suggest that these clones encode phyB phytochrome. However, the size of the deduced open reading frame of 1133 amino acids is smaller than the size of the other two phyB open reading frames characterized so far in higher plants, which contain 1171 or 1187 amino acids. The intron/exon structure within the coding region is conserved in phyA and phyB genes of various species. Southern blot analysis indicates that potato phyB is a single-copy gene. PhyB mRNA levels do not differ among different organs or different light regimes. Transcription initiation starts from two different start points which are 63 bp apart.
我们已经分离并测序了编码马铃薯光敏色素脱辅基蛋白的重叠基因组和cDNA克隆。基于推导的氨基酸序列，该序列与拟南芥phyB有77%的同一性，与马铃薯phyA开放阅读框有50%的同一性，我们认为这些克隆编码phyB光敏色素。然而，推导的1133个氨基酸的开放阅读框的大小小于目前在高等植物中鉴定的其他两个phyB开放阅读框的大小，后者包含1171或1187个氨基酸。编码区内的内含子/外显子结构在不同物种的phyA和phyB基因中是保守的。Southern印迹分析表明马铃薯phyB是单拷贝基因。不同器官或不同光照条件下，phyB mRNA水平没有差异。转录起始于两个相距63 bp的不同起始点。
