从新鲜植物组织、标本植物组织和木乃伊植物组织中提取毫克级 DNA。
Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.
机构信息
Department of Botany, University of Washington, 98195, Seattle, WA, U.S.A..
出版信息
Plant Mol Biol. 1985 Mar;5(2):69-76. doi: 10.1007/BF00020088.
We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.
我们开发了一种从毫克级植物组织中提取 DNA 的方法。每毫克组织的 DNA 产量在 0.3-200 纳克之间。讨论了影响产量的因素。新鲜组织、标本（22-118 岁）和木乃伊种子和胚胎（500 至超过 44600 岁）均被使用。所有尝试的组织（29 个物种的 57 种类型）都产生了可测量量的 DNA。在测试的所有情况下，都没有观察到 BamHI 或 EcoRI 限制酶的抑制作用。
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