Zúñiga Manuel, Pardo Isabel, Ferrer Sergi
Departament de Microbiologia, Universitat de València, 46100-Burjassot, Valencia, Spain.
Arch Microbiol. 2003 Nov;180(5):367-73. doi: 10.1007/s00203-003-0599-3. Epub 2003 Sep 20.
Conjugal transfer of plasmids pIP501 and its derivative pVA797 from Lactococcus lactis to Oenococcus oeni was assayed by filter mating. Plasmid pIP501 was transferred to a number of O. oeni strains whereas a single transconjugant of O. oeni M42 was recovered when pVA797 was used. Physical analysis of the transconjugant plasmids revealed that pIP501 and pVA797 underwent extensive deletions in O. oeni that affected the tra region (conjugal transfer) and SegB region (stability). All derivatives showed segregational instability in O. oeni, but were stably maintained in L. lactis. These differences correlated with the different plasmid copy numbers and the extent of deletions within the SegB region.
通过滤膜杂交试验检测了质粒pIP501及其衍生物pVA797从乳酸乳球菌向酒酒球菌的接合转移。质粒pIP501转移到了多个酒酒球菌菌株中，而当使用pVA797时，仅获得了一株酒酒球菌M42的转接合子。对转接合子质粒的物理分析表明，pIP501和pVA797在酒酒球菌中发生了广泛的缺失，这些缺失影响了转移区域（接合转移）和SegB区域（稳定性）。所有衍生物在酒酒球菌中均表现出分离不稳定性，但在乳酸乳球菌中能稳定维持。这些差异与不同的质粒拷贝数以及SegB区域内的缺失程度相关。
