Beltramo Charlotte, Oraby Mona, Bourel Gérald, Garmyn Dominique, Guzzo Jean
Laboratoire de Microbiologie, UMR-UB-INRA 1232, ENSBANA, 1 Esplanade Erasme, 21000 Dijon, France.
FEMS Microbiol Lett. 2004 Jul 1;236(1):53-60. doi: 10.1016/j.femsle.2004.05.029.
Despite the large number of techniques available for the transformation of bacteria, several species are still resistant to the introduction of foreign DNA. Oenococcus oeni are among the organisms that are particularly refractory to transformation. However, conjugal experiments from Lactococcus lactis to O. oeni with a new plasmid, pGID052, were performed via mobilization with success. This plasmid, a derivative of pORI19, encompasses: (i) the oriT of pIP501, which permitted the transfer to O. oeni, (ii) the replication genes of a native Leuconostoc citreum plasmid. Frequencies of 10(-7) conjugants per recipient were found. The transfer did not affect the structure of this low-copy-number plasmid. Moreover, pGID052 seems segregationally stable and could be used in the future as an expression vector.
尽管有大量可用于细菌转化的技术，但仍有几个物种对引入外源DNA具有抗性。酒类酒球菌是特别难以转化的生物之一。然而，通过用新质粒pGID052从乳酸乳球菌到酒类酒球菌进行的接合实验成功实现了转移。该质粒是pORI19的衍生物，包含：（i）pIP501的oriT，它允许转移到酒类酒球菌；（ii）天然柠檬明串珠菌质粒的复制基因。发现每个受体的接合子频率为10^(-7)。转移不影响这种低拷贝数质粒的结构。此外，pGID052似乎具有分离稳定性，未来可作为表达载体使用。
