Karnik Swapnil, Srinivasan Bhuvana, Nair Sheila
Department of Pathology, Christian Medical College and Hospital, Vellore, India.
Pathology. 2003 Jun;35(3):207-11. doi: 10.1080/0031302031000123164.
Immunohistochemistry (IHC) is known to aid in the diagnosis of Hodgkin's lymphoma (HL). We studied HL using an antibody panel for Reed-Sternberg cells (RSCs) to find which antibody would be most useful for identification of RSCs. We also studied the association of Epstein-Barr virus (EBV) latent membrane protein-1 (LMP-1) with HL in South India.
Lymph node biopsies of 100 cases of untreated HL were included in this study. Antibodies against CD15, CD30, CD3, CD20 (L26), CD45 (LCA), EMA and EBV LMP-1 were used for paraffin section IHC.
Of the 100 cases of HL, the RSCs stained with CD30 (93%), CD15 (67%), CD20 (17%) and CD3 (2%). EBV LMP-1 was positive in 82 (82%) cases, most often in the nodular sclerosis subtype, 43 (86%) cases.
(1) Of the panel of antibodies, CD30 was the most useful in identifying RSCs in classical HL. (2) EBV LMP-1 was demonstrated in 82% of all cases of HL and in 96% of childhood cases.
已知免疫组织化学（IHC）有助于霍奇金淋巴瘤（HL）的诊断。我们使用针对里德-施特恩伯格细胞（RSCs）的抗体组合研究HL，以找出哪种抗体对识别RSCs最有用。我们还研究了印度南部爱泼斯坦-巴尔病毒（EBV）潜伏膜蛋白-1（LMP-1）与HL的关联。
本研究纳入了100例未经治疗的HL患者的淋巴结活检标本。采用抗CD15、CD30、CD3、CD20（L26）、CD45（LCA）、EMA和EBV LMP-1抗体进行石蜡切片免疫组织化学检测。
在100例HL病例中，RSCs表达CD30的占93%，表达CD15的占67%，表达CD20的占17%，表达CD3的占2%。EBV LMP-1在82例（82%）病例中呈阳性，最常见于结节硬化亚型，共43例（86%）。
（1）在该抗体组合中，CD30对识别经典型HL中的RSCs最有用。（2）82%的HL病例以及96%的儿童HL病例中检测到EBV LMP-1。
