Kanavaros P, Sakalidou A, Gaulard P, Jiwa M, Delides G, Tzardi M, Kalmantis T, Lydaki E, Kazlaris E, Kalmanti M
Department of Pathology, University Hospital of Heraklion Crete, Greece.
Acta Morphol Hung. 1992;40(1-4):223-30.
Paraffin sections from 21 cases of Hodgkin's disease (HD), 28 cases of non-Hodgkin's lymphomas (NHL) and 34 cases of non-specific reactive lymphadenitides occurring in childhood were examined for the presence of the Epstein-Barr Virus (EBV)-encoded Latent Membrane Protein (LMP) using a double layer immunohistochemical method. LMP was detected in 12/21 (57%) cases of HD but not in NHL or reactive lymph nodes. LMP reactivity was restricted to Reed-Sternberg and Hodgkin's (HRS) cells in 4 of 9 (45%) cases of nodular sclerosis (NS), 6 of 9 (66%) cases of mixed cellularity (MC) and 2 of 2 (100%) cases of lymphocyte depletion (LD) while it was undetectable in the single case of lymphocyte predominance (LD) subtype. These results provide further evidence for an association between EBV and Hodgkin's disease, and they show that LMP expression occurs more frequently in the clinically more aggressive subtypes of HD. Furthermore, in view of the in vitro transforming potential of the LMP protein, the exclusive immunolocalization of LMP in HRS cells, suggests that EBV may be involved in the pathogenesis of a proportion of cases of HD.
采用双层免疫组化方法，检测了21例霍奇金病（HD）、28例非霍奇金淋巴瘤（NHL）和34例儿童期非特异性反应性淋巴结炎石蜡切片中EB病毒（EBV）编码的潜伏膜蛋白（LMP）的存在情况。在21例HD病例中有12例（57%）检测到LMP，而在NHL或反应性淋巴结中未检测到。在9例结节硬化型（NS）中的4例（45%）、9例混合细胞型（MC）中的6例（66%）以及2例淋巴细胞消减型（LD）中的2例（100%）中，LMP反应仅限于里德-斯腾伯格细胞和霍奇金细胞（HRS细胞），而在单一的淋巴细胞为主型（LP）亚型病例中未检测到。这些结果为EBV与霍奇金病之间的关联提供了进一步证据，并且表明LMP表达在临床上侵袭性更强的HD亚型中更频繁出现。此外，鉴于LMP蛋白的体外转化潜能，LMP在HRS细胞中的独特免疫定位提示EBV可能参与了一部分HD病例的发病机制。
