辣椒斑驳病毒和马铃薯Y病毒是不同病毒的证据：加利福尼亚州辣椒斑驳病毒分离株外壳蛋白和3'非翻译序列的分析

Evidence that pepper mottle virus and potato virus Y are distinct viruses: analyses of the coat protein and 3' untranslated sequence of a California isolate of pepper mottle virus.

作者信息

Vance V B, Jordan R, Edwardson J R, Christie R, Purcifull D E, Turpen T, Falk B

机构信息

Department of Biological Sciences, University of South Carolina, Columbia.

出版信息

Arch Virol Suppl. 1992;5:337-45. doi: 10.1007/978-3-7091-6920-9_36.

DOI:10.1007/978-3-7091-6920-9_36

PMID:1450760

Abstract

Pepper mottle virus (PepMoV) is a member of the large and complex genus Potyvirus, and is classically distinguished from other members of the genus by differential host range and cytopathology as well as serology of the coat protein and cytoplasmic inclusion body proteins. Here we report the deduced amino acid sequence of the coat protein of a California potyvirus identified by a variety of classical methods as PepMoV (PepMoV C). Comparison of the 3' untranslated nucleic acid sequence and the deduced coat-protein amino acid sequence of the PepMoV C isolate with those of PVY and other potyviruses indicates that PepMoV C is sufficiently diverged to be considered a distinct virus species. Thus, comparative sequence analyses of the PepMoV C isolate support earlier serological and biological evidence that PepMoV and PVY are distinct viruses.

摘要

辣椒斑驳病毒（PepMoV）是大型复杂的马铃薯Y病毒属的成员，传统上通过不同的寄主范围、细胞病理学以及外壳蛋白和细胞质内含体蛋白的血清学与该属的其他成员区分开来。在此，我们报告了一种加利福尼亚马铃薯Y病毒的外壳蛋白推导氨基酸序列，该病毒通过多种经典方法鉴定为PepMoV（PepMoV C）。将PepMoV C分离株的3'非翻译核酸序列和推导的外壳蛋白氨基酸序列与PVY及其他马铃薯Y病毒的相应序列进行比较，结果表明PepMoV C的差异足够大，可被视为一个独特的病毒种。因此，对PepMoV C分离株的比较序列分析支持了早期的血清学和生物学证据，即PepMoV和PVY是不同的病毒。