Wefels E, Sommer H, Salamini F, Rohde W
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Arch Virol. 1989;107(1-2):123-34. doi: 10.1007/BF01313884.
DNA was synthesized complementary to the RNA genome of potato virus Y (PVY; strain GO 16) and cloned into lambda vectors. The size of PVY-specific Eco RI-restricted cDNA ranged from 0.3 to approximately 22kb. Two of the cDNA clones each of which contained some 4kb of cDNA sequence starting from the 3'-polyadenylated terminus were characterized by sequence analysis. Presence of a single open reading frame suggests that PVY-specific proteins are synthesized as a polyprotein precursor. As with other sequenced potyvirus RNAs the gene for the PVY capsid protein CP is located next to the 3'-untranslated region followed by the genes for the putative RNA polymerase (nuclear inclusion protein NIb) and the virus-specific protease (nuclear inclusion protein NIa). The 3'-trailing sequence of the PVY strains cloned is highly homologous to the corresponding region of pepper mottle virus (PeMV) and suggests that PeMV is not a distinct member of the potyvirus group, but another strain of PVY.
合成了与马铃薯Y病毒（PVY；GO 16株系）RNA基因组互补的DNA，并克隆到λ载体中。PVY特异性Eco RI酶切cDNA的大小范围为0.3至约22kb。对两个cDNA克隆进行了序列分析，每个克隆包含从3'-聚腺苷酸化末端开始的约4kb cDNA序列。存在一个单一的开放阅读框表明PVY特异性蛋白作为多蛋白前体合成。与其他已测序的马铃薯Y病毒RNA一样，PVY衣壳蛋白CP基因位于3'-非翻译区旁边，接着是推定的RNA聚合酶（核内含体蛋白NIb）和病毒特异性蛋白酶（核内含体蛋白NIa）的基因。克隆的PVY株系的3'-末端序列与辣椒斑驳病毒（PeMV）的相应区域高度同源，这表明PeMV不是马铃薯Y病毒组的一个独特成员，而是PVY的另一个株系。
