García-Luque I, Ferrero M L, Rodríquez J M, Alonso E, de la Cruz A, Sanz A I, Vaquero C, Serra M T, Díaz-Ruíz J R
U.E.I. Fitopatología, Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.
Arch Virol. 1993;131(1-2):75-88. doi: 10.1007/BF01379081.
The nucleotide sequence of the coat protein genes and 3' non-coding regions of two different resistance-breaking tobamoviruses in pepper have been determined. The deduced coat protein of an Italian isolate of pepper mild mottle virus (PMMV-I) consists of 156 amino acids and its 3' non-coding region is 198 nucleotides long. They have been found to be very similar in sequence and structure to those previously reported for a Spanish isolate (PMMV-S). In contrast, a Dutch isolate termed P 11 codes for a coat protein of 160 amino acids and its 3' non-coding region is 291 nucleotides long, which may have arisen by duplication. The nucleotide and the predicted coat protein amino acid sequence analysis show that this isolate should be considered as a new virus within the tobamovirus group. The term paprika mild mottle virus (PaMMV) is proposed.
已确定了辣椒中两种不同的能突破抗性的烟草花叶病毒的外壳蛋白基因及3'非编码区的核苷酸序列。意大利辣椒轻斑驳病毒分离株(PMMV-I)推导的外壳蛋白由156个氨基酸组成,其3'非编码区长198个核苷酸。已发现它们在序列和结构上与先前报道的西班牙分离株(PMMV-S)非常相似。相比之下,一种名为P11的荷兰分离株编码的外壳蛋白有160个氨基酸,其3'非编码区长291个核苷酸,这可能是由重复产生的。核苷酸及预测的外壳蛋白氨基酸序列分析表明,该分离株应被视为烟草花叶病毒组内的一种新病毒。建议将其命名为辣椒轻斑驳病毒(PaMMV)。