Denoyer M, Lallement G, Collet A, Pernot-Marino I, Séréno D, Blanchet G
Centre de Recherches du Service de Santé des Armées, Unité de Neurotoxicologie, La Tronche, France.
Brain Res. 1992 Oct 2;592(1-2):157-62. doi: 10.1016/0006-8993(92)91670-a.
The effects of intraseptal application of atropine on c-fos proto-oncogene expression related to soman treatment were studied by immunohistochemistry for c-Fos-like proteins. In control rats, 2 h after the onset of convulsion, c-Fos-like immunoreactivity was intense in the piriform and entorhinal cortices, but also in the cingulate, frontoparietal and retrosplenial cortices. In addition, the staining was moderate in the hypothalamus, amygdala and fascia dentata. The intraseptal application of atropine, which prevented soman-induced convulsions, reduced or even blocked c-Fos-like protein production related to soman treatment. This inhibition of Fos induction was significant in most of the limbic structures but also in non-limbic areas. The data in this study strongly suggest that the cholinergic cells of the medial septal area play a key role in soman-induced seizures, and confirm that c-Fos-like protein induction is closely related to neuronal hyperactivity.
通过对c-Fos样蛋白进行免疫组织化学研究，探讨了隔区内注射阿托品对与梭曼处理相关的c-fos原癌基因表达的影响。在对照大鼠中，惊厥发作2小时后，梨状皮质和内嗅皮质出现强烈的c-Fos样免疫反应，扣带回、额顶叶皮质和压后皮质也出现这种反应。此外，下丘脑、杏仁核和齿状回的染色为中度。隔区内注射阿托品可预防梭曼诱导的惊厥，减少甚至阻断与梭曼处理相关的c-Fos样蛋白的产生。这种对Fos诱导的抑制在大多数边缘结构以及非边缘区域都很显著。本研究的数据强烈表明，内侧隔区的胆碱能细胞在梭曼诱导的癫痫发作中起关键作用，并证实c-Fos样蛋白的诱导与神经元活动亢进密切相关。
