Smielewska-Loś E
Department of Epizootiology and Veterinary Administration with Clinic, Faculty of Veterinary Medicine, Agricultural University, pl. Grunwaldzki 45, 50-366 Wrocław, Poland.
Pol J Vet Sci. 2003;6(3):229-34.
Tissues from 4 aborted polar foxes (3 samples of brain and 4 samples of liver) were selected for Toxoplasma gondii PCR assay. Positive results of serological tests of mothers and immunofluorescence test (IFT) of fetal organ smears were the criteria of sample selection. Five sets of primers designed from B1 gene and ITS1 sequences of T. gondii were used for detection of the parasite in fetal fox tissues. All used primer sets successfully amplified T. gondii DNA in PCR from organs which were positive by IFT. Single tube nested PCR also showed positive result from a sample negative by IFT, but this product was not confirmed. The studies showed usefullness of PCR for routine diagnosis of toxoplasmosis in carnivores.
选取4只流产的北极狐的组织（3份脑样本和4份肝样本）用于弓形虫PCR检测。以母体血清学检测阳性结果以及胎儿器官涂片免疫荧光试验（IFT）结果作为样本选择标准。从弓形虫B1基因和ITS1序列设计的5组引物用于检测北极狐胎儿组织中的寄生虫。所有使用的引物组均成功地从IFT呈阳性的器官的PCR中扩增出弓形虫DNA。单管巢式PCR也显示一份IFT呈阴性的样本结果为阳性，但该产物未得到确认。这些研究表明PCR在食肉动物弓形虫病常规诊断中具有实用性。
