Steuber S, Niu A, Bauer C, Reetz J, Roth A, Janitschke K
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin Berlin.
Dtsch Tierarztl Wochenschr. 1995 Feb;102(2):91-3.
A polymerase chain reaction was applied to detect Toxoplasma gondii DNA in placental and fetal tissue samples of 47 unselected ovine abortions of the lambing season 1990/91 (Baden-Württemberg, Rhineland, Hesse). For the amplification a 190 bp or 223 bp sequence of the B1-gene of T. gondii was selected as the target sequence. Both sequences were detected in five abortions. All positive results were immunohistochemically confirmed using the peroxidase antiperoxidase technique (PAP-staining). Thus, in Germany, too, T. gondii infection in sheep during pregnancy should be considered as a possible cause of abortions, particularly in case of abortions of unknown genesis.
应用聚合酶链反应检测了1990/91产羔季节（巴登-符腾堡州、莱茵兰、黑森州）47例未经挑选的绵羊流产胎盘和胎儿组织样本中的弓形虫DNA。为进行扩增，选择了弓形虫B1基因的190 bp或223 bp序列作为靶序列。在5例流产样本中检测到了这两种序列。所有阳性结果均采用过氧化物酶抗过氧化物酶技术（PAP染色）进行免疫组织化学确认。因此，在德国，孕期绵羊感染弓形虫也应被视为流产的一个可能原因，尤其是在病因不明的流产病例中。
