Luna C, Sansberro P, Mroginski L, Tarragó J
Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Sargento Cabral 2131, CC: 209. (3400) Corrientes, Argentina.
Biocell. 2003 Aug;27(2):205-12.
Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in 1/4 strength Murashige and Skoog medium (1/4 MS) plus 30 gr x L(-1) sucrose and supplemented with 4.4 microM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr x L(-1) sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in 1/4 MS (30 gr x L(-1) sucrose, 0.25% Phytagel) with 7.3 microM IBA and 2) 21 days in the same medium without IBA and 20 microM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation schedule can be implemented in breeding programs of Ilex dumosa.
对含一个腋芽的毛冬青（“yerba señorita”）茎段进行了微繁殖研究。在添加30 g·L⁻¹蔗糖并补充4.4 μM 苄氨基腺嘌呤的1/4强度Murashige和Skoog培养基（1/4 MS）中，六岁植株外植体很容易实现芽再生。通过在含1.5 g·L⁻¹蔗糖的类似成分新鲜培养基中继代培养，可实现再生芽的进一步增殖和伸长。芽的生根诱导分两步进行：1）在含7.3 μM 吲哚丁酸的1/4 MS（30 g·L⁻¹蔗糖，0.25% Phytagel）中培养7天；2）在不添加吲哚丁酸但添加20 μM尸胺的相同培养基中培养21天。再生植株成功移栽到土壤中。该微繁殖方案可应用于毛冬青的育种计划。
