Philomina N S, Rao J V
Department of Botany, S.V. University, Tirupati 517502, India.
Indian J Exp Biol. 2000 Jun;38(6):621-4.
Bud break and multiple shoots were induced in apical and axillary meristems derived from one month old seedlings of S. mukorossi on Murashige and Skoog (MS) medium supplemented with benzylamino purine (BAP) 0.4 microM or 0.8 microM alone. A combination of BAP and gibberellic acid (GA3) 0.4 microM and 2.8 microM produced elongated multiple shoots from both types of explants. Excised shoots were rooted on MS medium respectively with indole-3-butyric acid (IBA) 3.4 microM or 2.4 microM. The regenerated plantlets were successfully acclimatized and transferred to soil.
在添加了0.4微摩尔或0.8微摩尔苄氨基嘌呤（BAP）的Murashige和Skoog（MS）培养基上，从1月龄桑树苗的顶端和腋生分生组织中诱导出芽萌发和多个芽。0.4微摩尔BAP和2.8微摩尔赤霉素（GA3）的组合从两种外植体中产生了伸长的多个芽。切下的芽分别在含有3.4微摩尔或2.4微摩尔吲哚-3-丁酸（IBA）的MS培养基上生根。再生的小植株成功驯化并移栽到土壤中。
