不同技术在沙门氏菌病流行病学研究中的实用性。

Usefulness of different techniques in the study of the epidemiology of salmonellosis.

作者信息

Ruiz M, Rodríguez J C, Sirvent E, Escribano I, Cebrián L, Royo G

机构信息

Sección de Microbiología, Hospital General Universitario de Elche, Universidad Miguel Hernández, Elche, Spain.

出版信息

APMIS. 2003 Sep;111(9):848-56. doi: 10.1034/j.1600-0463.2003.1110903.x.

DOI:10.1034/j.1600-0463.2003.1110903.x

PMID:14510642

Abstract

Our objective was to evaluate the usefulness of serotyping, phagotyping, antibiotyping and RAPD (random amplified polymorphic DNA) in the study of the epidemiology of salmonellosis in Elche (Spain). We examined 1232 clinical isolates of non-typhoid Salmonella serotypes. Serotyping adequately differentiates 12.3% of the isolates and phagotyping raises this percentage to 37%. Antibiotyping is a suitable complement in certain circumstances, such as in the case of specific outbreaks. RAPD is a fast and simple technique which is within the reach of most laboratories, complementing the results obtained by antibiotyping and serotyping, in order to be able to control outbreaks of salmonellosis in everyday practice.

摘要

我们的目的是评估血清分型、噬菌体分型、抗生素分型和随机扩增多态性DNA（RAPD）在西班牙埃尔切沙门氏菌病流行病学研究中的作用。我们检测了1232株非伤寒沙门氏菌血清型的临床分离株。血清分型能充分区分12.3%的分离株，而噬菌体分型可将这一比例提高到37%。抗生素分型在某些情况下是一种合适的补充方法，例如在特定疫情爆发时。RAPD是一种快速简便的技术，大多数实验室都能采用，它可补充抗生素分型和血清分型所获得的结果，以便在日常实践中控制沙门氏菌病的爆发。