Calum Henrik, Moser Claus, Jensen Peter Østrup, Shirai Ryo, Høiby Niels
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
APMIS. 2003 Sep;111(9):891-7. doi: 10.1034/j.1600-0463.2003.1110907.x.
The purpose of the present study was to investigate whether NK cells from resistant C3H/HeN mice and susceptible BALB/c mice showed different release of cytokines and expression of surface molecules during chronic P. aeruginosa lung infection using alginate-embedded P. aeruginosa mimicking the infection in cystic fibrosis. Lung cell suspensions were depleted of lymphocytes by magnetic cell sorting. The concentrations of IFN-gamma, IL-1beta and GM-CSF were estimated by ELISA at day 1 and 2 after infection. Non-infected mice were used as controls. Flow cytometry was used to estimate the surface expression of the LFA-1 and Fc receptors on NK cells. At day 2, IFN-gamma levels increased in C3H/HeN mice but decreased in BALB/c mice. The GM-CSF levels increased only in the C3H/HeN mice at day 1 and 2. Surface expression of LFA-1 on the NK cells was higher in C3H/HeN mice at day 1 and 2. In contrast, the expression of Fc receptors was significantly lower on NK cells in C3H/HeN mice at day 1 and 2. In conclusion, the present results show phenotypic differences in NK cells in the two mice strains in chronic P. aeruginosa lung infection, indicating different modulating effects in the Th1/Th2 balance.
本研究的目的是利用模拟囊性纤维化感染的藻酸盐包埋铜绿假单胞菌，调查在慢性铜绿假单胞菌肺部感染期间，抗性C3H/HeN小鼠和易感BALB/c小鼠的自然杀伤（NK）细胞是否表现出细胞因子释放和表面分子表达的差异。通过磁性细胞分选去除肺细胞悬液中的淋巴细胞。在感染后第1天和第2天，采用酶联免疫吸附测定法（ELISA）评估干扰素-γ（IFN-γ）、白细胞介素-1β（IL-1β）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的浓度。未感染的小鼠用作对照。采用流式细胞术评估NK细胞上淋巴细胞功能相关抗原-1（LFA-1）和Fc受体的表面表达。在第2天，C3H/HeN小鼠的IFN-γ水平升高，而BALB/c小鼠的IFN-γ水平降低。GM-CSF水平仅在第1天和第2天在C3H/HeN小鼠中升高。在第1天和第2天，C3H/HeN小鼠NK细胞上LFA-1的表面表达较高。相反，在第1天和第2天，C3H/HeN小鼠NK细胞上Fc受体的表达明显较低。总之，目前的结果显示在慢性铜绿假单胞菌肺部感染中，两种小鼠品系的NK细胞存在表型差异，表明在Th1/Th2平衡中具有不同的调节作用。
