Moser C, Johansen H K, Song Z, Hougen H P, Rygaard J, Høiby N
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
APMIS. 1997 Nov;105(11):838-42.
The chronic Pseudomonas aeruginosa lung infection in cystic fibrosis (CF) is characterized by a pronounced antibody response and microcolonies surrounded by numerous polymorphonuclear neutrophils (PMN). Poor prognosis is correlated with a high antibody response to P. aeruginosa antigens. An animal model of this infection was established in two strains of mice: C3H/HeN and BALB/c, generally known as Th1 and Th2 responders, respectively, which were challenged with alginate-embedded P. aeruginosa. Mortality was significantly lower in C3H/HeN compared to BALB/c mice (p < 0.025). P. aeruginosa was cleared more efficiently in C3H/HeN mice and significantly more C3H/HeN mice showed normal lung histopathology (p < 0.02), and we found significantly fewer microabscesses in C3H/HeN mice than in BALB/c mice (p < 0.005). In supernatants from P. aeruginosa antigen and concanavalin A-stimulated spleen cells from the two strains of mice, the interferon-(IFN-) gamma levels were higher, whereas IL-4 levels were lower in C3H/HeN mice than in BALB/c mice. The implications of these findings for CF patients with chronic P. aeruginosa lung infection are discussed.
囊性纤维化（CF）患者的慢性铜绿假单胞菌肺部感染的特征是有明显的抗体反应以及被大量多形核中性粒细胞（PMN）包围的微菌落。预后不良与对铜绿假单胞菌抗原的高抗体反应相关。在两种小鼠品系中建立了这种感染的动物模型：C3H/HeN和BALB/c，通常分别称为Th1和Th2反应小鼠，用藻酸盐包埋的铜绿假单胞菌对其进行攻击。与BALB/c小鼠相比，C3H/HeN小鼠的死亡率显著降低（p<0.025）。C3H/HeN小鼠体内的铜绿假单胞菌清除效率更高，显著更多的C3H/HeN小鼠表现出正常的肺组织病理学（p<0.02），并且我们发现C3H/HeN小鼠中的微脓肿明显少于BALB/c小鼠（p<0.005）。在来自两种小鼠品系的铜绿假单胞菌抗原和伴刀豆球蛋白A刺激的脾细胞的上清液中，C3H/HeN小鼠的干扰素-（IFN-）γ水平较高，而IL-4水平低于BALB/c小鼠。讨论了这些发现对患有慢性铜绿假单胞菌肺部感染的CF患者的意义。
