Kaiser M G, Lakshmanan N, Arthur J A, O'Sullivan N P, Lamont S J
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Anim Genet. 2003 Oct;34(5):334-8. doi: 10.1046/j.1365-2052.2003.01022.x.
A potential limitation of the use of a dominant molecular marker system such as DNA fingerprinting (DFP) is the inability to distinguish homozygous from heterozygous allele state in an individual, and a resulting inaccuracy in estimating effects of the marker alleles. The objective of this study was to accurately estimate the effect of DFP markers on egg-production traits. A BC1 population was produced from two distinct layer lines. Four DFP bands, each originating predominantly in one of the two parental lines, were evaluated for linkage with egg-production quantitative trait loci in the BC1 population. The egg-production traits consisted of eight early period and seven late period measurements. Eight marker-trait linkages were identified out of 60 total statistical tests. By utilizing information on frequency of DFP bands in two parental lines, selecting F1 sires with DFP bands present, and backcrossing to the line lacking these bands, the population design allowed definitive identification of the DFP zygosity in the BC1 resource population hens. In this manner, accurate estimates of marker allele effects on egg-production traits were obtained from the dominant marker system of DNA fingerprinting.
使用诸如DNA指纹图谱（DFP）这样的显性分子标记系统的一个潜在局限性是无法区分个体中的纯合等位基因状态和杂合等位基因状态，从而在估计标记等位基因的效应时产生不准确的结果。本研究的目的是准确估计DFP标记对产蛋性状的影响。从两个不同的蛋鸡品系培育出了一个BC1群体。评估了四个DFP条带（每个条带主要起源于两个亲本系之一）与BC1群体中产蛋数量性状基因座的连锁关系。产蛋性状包括八个早期测量值和七个晚期测量值。在总共60次统计检验中鉴定出了八个标记-性状连锁关系。通过利用两个亲本系中DFP条带的频率信息，选择具有DFP条带的F1父本，并与缺乏这些条带的品系回交，这种群体设计使得能够明确鉴定BC1资源群体母鸡中的DFP纯合性。通过这种方式，从DNA指纹图谱的显性标记系统中获得了标记等位基因对产蛋性状影响的准确估计值。
