Dunnington E A, Stallard L C, Siegel P B, Hillel J
Poultry Science Department, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Poult Sci. 1993 Jun;72(6):1000-4. doi: 10.3382/ps.0721000.
This study assessed the influence of background genome on expression of genes linked to DNA fingerprint (DFP) bands in chickens. Two experimental lines of White Plymouth Rocks previously selected for high or low 8-wk body weight were crossed to produce two F1 males that served as heads of two sire families. Each of these sires was mated to three hens from an unrelated White Leghorn population to produce progeny in which quantitative traits of 4-, 8-, and 12-wk body weight and shank length at 12 wk were measured. The DFP patterns were produced for all individuals in the study. For the 12 sire-specific DFP bands (common to both F1 sires but not found in the dams), each offspring was classified as having or not having each band. Then, an analysis of variance was conducted for each DFP sire band with sex, hatch, dam family, and presence or absence of the sire band as main effects. Interactions between dam family and presence or absence of sire band were also tested. Of 48 possible analyses of variance (12 sire bands by 4 quantitative traits), 3 resulted in significant effects due to sire band, and 2 indicated significant interactions. Thus, associations of a DFP band and a gene coding for a quantitative trait were present, but, in some cases, the expression of the trait differed, depending on the dam family in which it occurred. These data suggest that associations between DFP bands and quantitative traits may not be consistent in different genetic backgrounds.
本研究评估了背景基因组对鸡中与DNA指纹（DFP）条带相关基因表达的影响。先前选择了8周龄体重高或低的两个白普利茅斯洛克实验品系进行杂交，产生了两只F1雄性，作为两个父系家族的族长。每个父系与来自无关白来航群体的三只母鸡交配，以产生后代，测量其4周龄、8周龄和12周龄体重以及12周龄胫长的数量性状。对研究中的所有个体进行了DFP模式分析。对于12个父系特异性DFP条带（两个F1父系共有，但在母系中未发现），每个后代被分类为具有或不具有每个条带。然后，以性别、孵化、母系家族以及父系条带的有无为主要效应，对每个DFP父系条带进行方差分析。还测试了母系家族与父系条带有无之间的相互作用。在48种可能的方差分析（12个父系条带×4个数量性状）中，有3个因父系条带而产生显著效应，2个表明存在显著相互作用。因此，存在DFP条带与编码数量性状的基因之间的关联，但在某些情况下，性状的表达因所在的母系家族而异。这些数据表明，DFP条带与数量性状之间的关联在不同遗传背景下可能不一致。
