人类甲状腺癌和腺瘤中的蛋白激酶C活性

Protein kinase C activity in human thyroid carcinoma and adenoma.

作者信息

Hatada T, Sakanoue Y, Kusunoki M, Kobayashi A, Utsunomiya J

机构信息

Second Department of Surgery, Hyogo College of Medicine, Japan.

出版信息

Cancer. 1992 Dec 15;70(12):2918-22. doi: 10.1002/1097-0142(19921215)70:12<2918::aid-cncr2820701231>3.0.co;2-5.

DOI:10.1002/1097-0142(19921215)70:12<2918::aid-cncr2820701231>3.0.co;2-5

PMID:1451074

Abstract

Protein kinase C (PKC) activity was examined in the cytosolic and particulate fractions of homogenates obtained from 12 papillary thyroid carcinomas, 12 follicular thyroid adenomas, and the adjacent normal thyroid tissue. Particulate PKC activity was elevated significantly in thyroid carcinomas compared with normal thyroid tissue (P < 0.01) and adenomas (P < 0.05). By contrast, cytosolic PKC activity of carcinomas and adenomas was lower significantly than that of normal thyroid tissue (P < 0.01). The percentage of particulate PKC activity in carcinoma and adenoma was higher than in normal thyroid tissue (carcinoma, P < 0.001; adenoma, P < 0.01). The average particulate PKC activity of carcinomas more than 3 cm in diameter was significantly lower than that of carcinomas less than or equal to 3 cm in diameter (P < 0.05). The average cytosolic PKC activity of carcinomas more than 3 cm also was lower significantly than that of smaller carcinomas (P < 0.05). These results suggest that alterations in PKC activity may be important in the development of papillary thyroid cancer.

摘要

在取自12例甲状腺乳头状癌、12例甲状腺滤泡性腺瘤及相邻正常甲状腺组织的匀浆的胞质和颗粒部分检测蛋白激酶C（PKC）活性。与正常甲状腺组织（P < 0.01）和腺瘤（P < 0.05）相比，甲状腺癌中的颗粒性PKC活性显著升高。相比之下，癌组织和腺瘤的胞质PKC活性显著低于正常甲状腺组织（P < 0.01）。癌组织和腺瘤中颗粒性PKC活性的百分比高于正常甲状腺组织（癌，P < 0.001；腺瘤，P < 0.01）。直径大于3 cm的癌组织的平均颗粒性PKC活性显著低于直径小于或等于3 cm的癌组织（P < 0.05）。直径大于3 cm的癌组织的平均胞质PKC活性也显著低于较小癌组织（P < 0.05）。这些结果表明，PKC活性的改变可能在甲状腺乳头状癌的发生发展中起重要作用。