Bindu L, Balaram P, Mathew A, Remani P, Bhattathiri V N, Nair M K
Division of Cancer Research, Regional Cancer Centre Division of Cancer Epidemiology and Clinical Research, Trivandrum, India.
Cytopathology. 2003 Oct;14(5):287-93. doi: 10.1046/j.1365-2303.2003.00059.x.
The aim of this study was to see whether serial cytological evaluation of various cellular abnormalities in tumours from patients receiving fractionated radiotherapy can predict radio-response in oral carcinoma. Cytological assessment was carried out in scrape smears collected prior to and during the course of radiotherapy in 68 patients with squamous cell carcinoma of the oral cavity planned for radical radiotherapy with accelerated fraction schedule. Smears were evaluated for a set of 15 radiation-induced cellular abnormalities. The relationship between the cellular alterations and the cumulative radiation dose was analysed by Kruskal-Wallis one-way anova. The results showed that among the various quantifiable changes that occur in irradiated cancer cells, karyolysis, karyorrhexis, pyknosis, cytolysis, multinucleation, micronucleation and nuclear budding show significant increase depending on the dose of radiation. The radio-resistant group of patients exhibited a lesser degree of change compared with the radio-sensitive group. This suggests that radio-resistance may be due to the defective induction of cell damage and that these cytological features may have potential use as predictive markers of radio-sensitivity in oral carcinoma.
本研究的目的是观察接受分次放疗患者肿瘤中各种细胞异常的系列细胞学评估能否预测口腔癌的放射反应。对68例计划采用加速分割方案进行根治性放疗的口腔鳞状细胞癌患者放疗前及放疗过程中采集的刮片进行细胞学评估。对一组15种辐射诱导的细胞异常进行涂片评估。采用Kruskal-Wallis单向方差分析分析细胞改变与累积辐射剂量之间的关系。结果表明，在受照射癌细胞中发生的各种可量化变化中，核溶解、核碎裂、核固缩、细胞溶解、多核化、微核化和核出芽随辐射剂量的增加而显著增加。与放射敏感组相比，放射抵抗组患者的变化程度较小。这表明放射抵抗可能是由于细胞损伤诱导缺陷所致，并且这些细胞学特征可能具有作为口腔癌放射敏感性预测标志物的潜在用途。
