Patil Vinit, Baad Rajendra, Gudur Anand, Vibhute Nupura, Belgaumi Uzma, Kadashetti Vidya
Department of Oral Pathology and Microbiology, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed University, Karad, Telangana, India, e-mail:
Department of Oral Pathology and Microbiology, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed University, Karad, Telangana, India.
J Contemp Dent Pract. 2018 Dec 1;19(12):1474-1479.
To assess various cytological changes for predicting radiosensitivity of oral squamous cell carcinoma by exfoliative cytology.
Histologically proven 30 cases of oral squamous cell carcinoma who underwent fractionated radiotherapy in a dose of 45-60 Gy in 5 fractions/week were enrolled in the study. The exfoliative cytology smear was evaluated on lesional and adjacent oral mucosa before radiotherapy, during radiotherapy (8 and 11th fraction) and post radiotherapy (4, 6 and 8 weeks). Various parameters like multinucleation, cellular enlargement, nuclear enlargement, cytoplasmic vacuolation, cytoplasmic granulation, leukocytic infiltration were evaluated.
Statistical significant values were seen in the inter-group comparison of all the parameters when compared adjacent mucosa and normal mucosa for leukocytic infiltration in pretreatment smear.
The study showed that radiation-induced cytological changes in oral squamous cell carcinoma have a significant dose-related increase. This dose-response relationship and the high intratumoral variations suggest that serial assay of these changes has potential use for radiosensitivity prediction.
Radiosensitivity prediction can be evaluated by means of cytological smears in one stop crisis centre (OSCC) individuals subjected to fractionated radiotherapy by evaluating the cytological parameters.
通过脱落细胞学评估口腔鳞状细胞癌的各种细胞学变化以预测放射敏感性。
选取30例经组织学证实的口腔鳞状细胞癌患者，这些患者接受每周5次、总剂量为45 - 60 Gy的分次放疗。在放疗前、放疗期间（第8次和第11次分次放疗时）以及放疗后（4、6和8周）对病变及相邻口腔黏膜的脱落细胞学涂片进行评估。评估了多核化、细胞增大、核增大、细胞质空泡化、细胞质颗粒化、白细胞浸润等各种参数。
在预处理涂片中，比较相邻黏膜和正常黏膜的白细胞浸润情况时，所有参数的组间比较均显示出统计学显著差异。
该研究表明口腔鳞状细胞癌中辐射诱导的细胞学变化有显著的剂量相关增加。这种剂量反应关系以及肿瘤内的高度变异性表明，对这些变化进行系列检测可能有助于预测放射敏感性。
通过评估细胞学参数，可在接受分次放疗的口腔鳞状细胞癌（OSCC）患者的一站式危机中心通过细胞学涂片评估放射敏感性。
