Pala Laura, Mannucci Edoardo, Pezzatini Anna, Ciani Silvia, Sardi Jacopo, Raimondi Laura, Ognibene Agostino, Cappadona Angela, Vannelli Barbara G, Rotella Carlo M
Department of Clinical, Pathophysiology, University of Florence, Italy
Biochem Biophys Res Commun. 2003 Oct 10;310(1):28-31. doi: 10.1016/j.bbrc.2003.08.111.
Glucagon-like peptide-1 (GLP-1), a meal-stimulated gastrointestinal insulinotropic hormone inactivated by dipeptidyl peptidase-IV (DPP-IV), is reduced in type 2 diabetic patients. The present study shows that 2-week exposure of human glomerular endothelial cells to high glucose (22 mM) determines a highly significant increase in DPP-IV activity and mRNA expression, which cannot be entirely accounted for by hyperosmolarity. On the other hand, incubation of purified DPP-IV in a buffer solution added with high glucose does not affect enzyme activity. These results suggest that high glucose increases expression and activity of DPP-IV, possibly contributing to GLP-1 reduction in type 2 diabetic patients.
