DeFilippis Victor, Raggo Camilo, Moses Ashlee, Früh Klaus
Vaccine and Gene Therapy Institute, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
Trends Biotechnol. 2003 Oct;21(10):452-7. doi: 10.1016/S0167-7799(03)00207-5.
Virology research and antiviral drug discovery are poised to benefit from the post-genomic revolution for three main reasons. First, viruses need the host to replicate and are therefore vulnerable to inhibition of cellular pathways. Knowledge of complete genomic sequences of both virus and host now permits the study of this interplay on a global scale. Combining transcriptomics and proteomics with large-scale gene knockdown experiments will enable the identification of novel antiviral targets. Second, massive parallel assay systems, such as DNA microarrays, which define the post-genomic era, will facilitate viral diagnostics. Third, the combination of genetics with genomics will enable the analysis of viral mutants and strains on an unprecedented scale. The dramatic effects of viral infection on host cell transcriptional patterns have been well-documented and will be briefly highlighted. In addition, we discuss recent trends that apply functional genomics methods to the discovery of new targets and therapies for viral disease.
病毒学研究和抗病毒药物研发有望从后基因组革命中受益，主要有三个原因。首先，病毒需要宿主进行复制，因此易受细胞途径抑制的影响。现在，病毒和宿主完整基因组序列的知识使得在全球范围内研究这种相互作用成为可能。将转录组学和蛋白质组学与大规模基因敲除实验相结合，将能够识别新的抗病毒靶点。其次，大规模平行检测系统，如定义后基因组时代的DNA微阵列，将促进病毒诊断。第三，遗传学与基因组学的结合将使前所未有的大规模病毒突变体和毒株分析成为可能。病毒感染对宿主细胞转录模式的显著影响已有充分记录，将在此简要强调。此外，我们还将讨论应用功能基因组学方法发现病毒疾病新靶点和新疗法的最新趋势。
