Zhang Rong
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Methods Mol Biol. 2023;2585:105-117. doi: 10.1007/978-1-0716-2760-0_11.
The mosquito-borne West Nile virus (WNV) poses a great threat to public health as no vaccine or specific antiviral treatment is available. Exploring virus-host interactions, specifically host factors that are required for virus infection, is important for better understanding the biology, pathogenesis, and transmission of WNV. Such essential host factors may also represent antiviral targets. The development of CRISPR technology has provided a powerful and convenient tool to perturbate host gene expression, allowing for unbiased, genome-wide screens of host factors for virus infection. Here we describe the necessary steps for performing a CRISPR knockout screen, which can also be applied to other viruses, to identify host factors critical for WNV infection.
蚊媒传播的西尼罗河病毒（WNV）对公共卫生构成了巨大威胁，因为目前尚无疫苗或特异性抗病毒治疗方法。探索病毒与宿主的相互作用，特别是病毒感染所需的宿主因子，对于更好地理解WNV的生物学特性、发病机制和传播至关重要。这些必需的宿主因子也可能代表抗病毒靶点。CRISPR技术的发展提供了一个强大且便捷的工具来干扰宿主基因表达，从而能够对病毒感染的宿主因子进行无偏倚的全基因组筛选。在此，我们描述了进行CRISPR基因敲除筛选的必要步骤，该步骤也可应用于其他病毒，以鉴定对WNV感染至关重要的宿主因子。
