Lobit P, Génard M, Wu B H, Soing P, Habib R
Ctifl, Centre de Balandran, 30127 Bellegarde, France.
J Exp Bot. 2003 Nov;54(392):2489-501. doi: 10.1093/jxb/erg264. Epub 2003 Sep 25.
Citrate production and degradation during the last stage of fruit development were modelled by representing the fluxes through the enzymes of the citrate cycle and the malic enzyme, the transport of metabolites between the cytosol and the mitochondria, and the stoichiometry equations that relate these reactions. After solving the corresponding system of equations, the rate of citrate synthesis (or degradation) was expressed as a simple function of temperature, mesocarp weight, and respiration. The model was applied to peach fruit, and its parameters were estimated from the data of a 2-year field experiment. The predictions of the model were in agreement with experimental data. Simulations were made to analyse the responses to variations of temperature and fruit growth. Increasing fruit growth before stone hardening stimulated citrate production, while increasing fruit growth after stone hardening reduced it. Delaying the date at which the maximum growth rate was reached enhanced citrate production during most of the period. In the last weeks before harvest, increasing temperature depressed citrate production, while, at the beginning of the period studied, it enhanced it.
通过描述柠檬酸循环酶和苹果酸酶的通量、细胞质与线粒体之间代谢物的转运以及关联这些反应的化学计量方程，对果实发育最后阶段的柠檬酸生成和降解进行了建模。求解相应方程组后，柠檬酸合成（或降解）速率表示为温度、中果皮重量和呼吸作用的简单函数。该模型应用于桃果实，并根据两年田间试验数据估算其参数。模型预测结果与实验数据相符。通过模拟分析了对温度变化和果实生长的响应。硬核前果实生长增加会刺激柠檬酸生成，而硬核后果实生长增加则会减少柠檬酸生成。延迟达到最大生长速率的日期会在大部分时期提高柠檬酸生成。在收获前的最后几周，温度升高会抑制柠檬酸生成，而在研究期开始时，温度升高会促进柠檬酸生成。
