Klymiuk Nikolai, Müller Mathias, Brem Gottfried, Aigner Bernhard
ApoGene Biotechnologie, D-86567 Hilgertshausen, Germany.
J Virol. 2003 Oct;77(20):11268-73. doi: 10.1128/jvi.77.20.11268-11273.2003.
Endogenous retrovirus (ERV) sequences have been found in all mammals. In vitro and in vivo experiments revealed ERV activation and cross-species infection in several species. Sheep (Ovis aries) are used for various biotechnological purposes; however, they have not yet been comprehensively screened for ERV sequences. Therefore, the aim of the study was to classify the ERV sequences in the ovine genome (OERV) by analyzing the retroviral pro-pol sequences. Three OERV beta families and nine OERV gamma families were revealed. Novel open reading frames (ORF) in the amplified proviral fragment were found in one OERV beta family and two OERV gamma families. Hybrid OERV produced by putative recombination events were not detected. Quantitative analysis of the OERV sequences in the ovine genome revealed no relevant variations in the endogenous retroviral loads of different breeds. Expression analysis of different tissues from fetal and pregnant sheep detected mRNA from both gammaretrovirus families, showing ORF fragments. Thus, the release of retroviruses from sheep cells cannot be excluded.
在所有哺乳动物中都发现了内源性逆转录病毒（ERV）序列。体外和体内实验揭示了几种物种中的ERV激活和跨物种感染。绵羊（Ovis aries）被用于各种生物技术目的；然而，尚未对它们进行全面的ERV序列筛查。因此，本研究的目的是通过分析逆转录病毒的前多聚蛋白序列对绵羊基因组中的ERV序列（OERV）进行分类。发现了三个OERVβ家族和九个OERVγ家族。在一个OERVβ家族和两个OERVγ家族的扩增前病毒片段中发现了新的开放阅读框（ORF）。未检测到由假定重组事件产生的杂交OERV。对绵羊基因组中OERV序列的定量分析表明，不同品种的内源性逆转录病毒载量没有相关差异。对胎儿和怀孕绵羊不同组织的表达分析检测到来自两个γ逆转录病毒家族的mRNA，显示出ORF片段。因此，不能排除绵羊细胞释放逆转录病毒的可能性。
