Xiao Rui, Park Kwangha, Lee Hoontaek, Kim Jinhoi, Park Chankyu
Department of Animal Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 143-701, South Korea.
J Virol. 2008 Jan;82(1):582-7. doi: 10.1128/JVI.01451-07. Epub 2007 Oct 24.
The aim of this study was to identify the endogenous retrovirus (ERV) sequences in a bovine genome. We subjected bovine genomic DNA to PCR with degenerate or ovine ERV (OERV) family-specific primers that aimed to amplify the retroviral pro/pol region. Sequence analysis of 113 clones obtained by PCR revealed that 69 were of retroviral origin. On the basis of the OERV classification system, these clones from degenerate PCR could be divided into the beta3, gamma4, and gamma9 families. PCR with OERV family-specific primers revealed an additional ERV that was classified into the bovine endogenous retrovirus (BERV) gamma7 family. In conclusion, here we report the results of a genome scale study of the BERV. Our study shows that the ERV family expansion in cattle may be somewhat limited, while more diverse family members of ERVs have been reported from other artiodactyls, such as pigs and sheep.
本研究的目的是鉴定牛基因组中的内源性逆转录病毒（ERV）序列。我们用简并引物或绵羊ERV（OERV）家族特异性引物对牛基因组DNA进行PCR，旨在扩增逆转录病毒的pro/pol区域。对通过PCR获得的113个克隆进行序列分析，结果显示其中69个来源于逆转录病毒。根据OERV分类系统，这些来自简并PCR的克隆可分为beta3、gamma4和gamma9家族。用OERV家族特异性引物进行PCR，还发现了另一种ERV，它被归类为牛内源性逆转录病毒（BERV）gamma7家族。总之，我们在此报告BERV的全基因组规模研究结果。我们的研究表明，牛中ERV家族的扩张可能在一定程度上受到限制，而在其他偶蹄目动物，如猪和绵羊中，已报道有更多样化的ERV家族成员。
