Brugerolle G, Mignot J-P
Biologie des Protistes, Unité Mixte de Recherche 6023, Centre National de la Recherche Scientifique, Université Blaise Pascal, Aubière, France.
Protoplasma. 2003 Sep;222(1-2):13-21. doi: 10.1007/s00709-003-0016-4.
An ultrastructure study of the rhizoplast in Synura petersenii, Mallomonas fastigiata, and M. insignis shows that it consists of 15-20 striated rootlets that form a claw or an incomplete cone over the nucleus. These rootlets course along one face of the nucleus between the nuclear membrane and the cis-face of the Golgi stack of cisternae. They converge and merge above the nucleus, forming a stub attached to the proximal section of the two basal bodies. These cross-striated rootlets are composed of closely packed longitudinal microfibrils. By immunofluorescence, the basal bodies and the rootlets forming the claw were decorated by the anti-centrin monoclonal antibody ICL19 raised against the Paramecium tetraurelia acidic centrin protein and by two antibodies raised against the striated parabasal and costal striated fibres of trichomonads. Only the anti-centrin monoclonal antibody 20H5 raised against Chlamydomonas reinhardtii centrin strongly labelled the 20-22 kDa protein bands from the extracted cytoskeleton of S. petersenii by immunoblotting. Electron micrographs of mitosis in S. petersenii cells revealed that the segregated pairs of basal bodies are linked by the striated rootlets of the rhizoplast to the poles of the mitotic spindle. The spindle microtubules arise perpendicularly from the striated rootlets of the basal body-nucleus connector forming the centrosome. In conclusion, in these cells there is a basal body-nucleus connector similar to that of C. reinhardtii and other chlorophytes. It contains centrin proteins, it is involved in the linkage of the basal bodies to the nucleus and is a component of the spindle pole body or centrosome in the dividing cell.
对彼得森合尾藻、直立鱼鳞藻和奇异鱼鳞藻中根丝体的超微结构研究表明，它由15 - 20条横纹小根组成，这些小根在细胞核上方形成一个爪状结构或不完整的锥体。这些小根沿着细胞核的一个面，在核膜和高尔基潴泡堆叠的顺面之间延伸。它们在细胞核上方汇聚并融合，形成一个附着在两个基体近端部分的短柄。这些横纹小根由紧密排列的纵向微纤丝组成。通过免疫荧光法，针对嗜热四膜虫酸性中心蛋白产生的抗中心蛋白单克隆抗体ICL19以及针对毛滴虫横纹副基体和肋状横纹纤维产生的两种抗体，对形成爪状结构的基体和小根进行了标记。只有针对莱茵衣藻中心蛋白产生的抗中心蛋白单克隆抗体20H5通过免疫印迹法强烈标记了从彼得森合尾藻提取的细胞骨架中的20 - 22 kDa蛋白条带。彼得森合尾藻细胞有丝分裂的电子显微照片显示，分离的基体对通过根丝体的横纹小根与有丝分裂纺锤体的两极相连。纺锤体微管垂直地从形成中心体的基体 - 细胞核连接体的横纹小根中产生。总之，在这些细胞中存在一种类似于莱茵衣藻和其他绿藻的基体 - 细胞核连接体。它含有中心蛋白，参与基体与细胞核的连接，并且是分裂细胞中纺锤极体或中心体的一个组成部分。
