Corrêa Gladys, Morgado-Diaz José Andres, Benchimol Marlene
Laboratório de Ultraestrutura Celular, Universidade Santa Ursula, Rua Jornalista Orlando Dantas, 59 Botafogo, CEP 22231-010, Rio de Janeiro, Brazil.
FEMS Microbiol Lett. 2004 Apr 1;233(1):91-6. doi: 10.1016/j.femsle.2004.01.043.
Giardia lamblia is a multiflagellar parasite and one of the earliest diverging eukaryotic cells. It possesses a complex cytoskeleton based on different groups of microtubular structures - a ventral adhesive disc, four pairs of flagella, a median body and funis. Centrin is an important member of the EF-hand family of calcium-binding proteins, and it is known to show calcium-sensitive contractile behaviour. In the present study, we performed an ultrastructural localization of centrin in G. lamblia using several monoclonal antibodies to centrin. Microtubular structures such as the basal bodies, all the flagella axonemes, the adhesive disc, funis, and the median bodies presented positive labelling to centrin. In addition, the dense rods also demonstrated positive labelling. These results show that centrin is located in key positions related to microtubules. The role of centrin in these dynamic regions is discussed.
蓝氏贾第鞭毛虫是一种多鞭毛寄生虫，也是最早分化的真核细胞之一。它拥有基于不同微管结构组的复杂细胞骨架——一个腹侧黏附盘、四对鞭毛、一个中体和索。中心蛋白是钙结合蛋白EF手家族的重要成员，已知其表现出对钙敏感的收缩行为。在本研究中，我们使用几种针对中心蛋白的单克隆抗体对蓝氏贾第鞭毛虫中的中心蛋白进行了超微结构定位。诸如基体、所有鞭毛轴丝、黏附盘、索和中体等微管结构对中心蛋白呈现阳性标记。此外，致密棒也显示出阳性标记。这些结果表明中心蛋白位于与微管相关的关键位置。本文讨论了中心蛋白在这些动态区域中的作用。
