Cheng Dong, Zheng Xin-Min, Li Shi-Wen, Yang Zhi-Wei, Hu Li-Quan
Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.
Zhonghua Nan Ke Xue. 2003 Aug;9(5):347-9.
To investigate the expression of FasL in rat cryptorchidism and its significance.
Twenty-four male SD rats (22-day old) were randomly divided into two groups: unilateral cryptorchid group (n = 12) and pseudo-operation group (n = 12). When the rats were 110-day old, blood samples were taken and the rats were killed for analysis. Immunohistochemical method (SP) was used to detect FasL expression in testes and ELISA method to detect serum antisperm antibody (AsAb).
The positive FasL expression rates in cryptorchid and contralateral testes were significantly higher than those in pseudo-operation group (P < 0.001). The serum AsAb positive rates in the cryptorchid group and the pseudo-operation group were 41.7% and 0, respectively, with significant difference(P < 0.01).
FasL expression upregulating in both testes of the unilateral cryptorchid rat may be a protective response of the testis to autoimmunity.
探讨FasL在大鼠隐睾中的表达及其意义。
将24只22日龄雄性SD大鼠随机分为两组：单侧隐睾组（n = 12）和假手术组（n = 12）。大鼠110日龄时，采集血样并处死大鼠进行分析。采用免疫组织化学方法（SP）检测睾丸中FasL表达，采用酶联免疫吸附测定法检测血清抗精子抗体（AsAb）。
隐睾侧及对侧睾丸FasL阳性表达率均显著高于假手术组（P < 0.001）。隐睾组和假手术组血清AsAb阳性率分别为41.7%和0，差异有统计学意义（P < 0.01）。
单侧隐睾大鼠双侧睾丸中FasL表达上调可能是睾丸对自身免疫的一种保护反应。
