Trezise Ann E O, Palazon Luis, Davies Wayne L, Colledge William H
School of Biomedical Sciences, University of Queensland, Brisbane, Qld 4072, Australia.
Curr Opin Mol Ther. 2003 Aug;5(4):397-404.
The use of electric pulses to deliver therapeutic molecules to tissues and organs in vivo is a rapidly growing field of research. Electrotransfer can be used to deliver a wide range of potentially therapeutic agents, including drugs, proteins, oligonucleotides, RNA and DNA. Optimization of this approach depends upon a number of parameters such as target organ accessibility, cell turnover, microelectrode design, electric pulsing protocols and the physiological response to the therapeutic agent. Many organs have been successfully transfected by electroporation, including skin, liver, skeletal and cardiac muscle, male and female germ cells, artery, gut, kidney, retinal ganglion cells, cornea, spinal cord, joint synovium and brain. Electrotransfer technology is relevant in a variety of research and clinical settings including cancer therapy, modulation of pathogenic immune reactions, delivery of therapeutic proteins and drugs, and the identification of drug targets by the modulation of normal gene expression. This, together with the capacity to deliver very large DNA constructs, greatly expands the research and clinical applications of in vivo DNA electrotransfer.
利用电脉冲在体内将治疗性分子递送至组织和器官是一个快速发展的研究领域。电转染可用于递送多种潜在的治疗剂，包括药物、蛋白质、寡核苷酸、RNA和DNA。这种方法的优化取决于许多参数，如靶器官的可及性、细胞更新、微电极设计、电脉冲方案以及对治疗剂的生理反应。许多器官已通过电穿孔成功转染，包括皮肤、肝脏、骨骼肌和心肌、雄性和雌性生殖细胞、动脉、肠道、肾脏、视网膜神经节细胞、角膜、脊髓、关节滑膜和大脑。电转染技术在多种研究和临床环境中都具有相关性，包括癌症治疗、致病性免疫反应的调节、治疗性蛋白质和药物的递送，以及通过调节正常基因表达来鉴定药物靶点。这与递送非常大的DNA构建体的能力一起，极大地扩展了体内DNA电转染的研究和临床应用。
