Shang Longan, Jiang Min, Chang Ho Nam
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea.
Biotechnol Lett. 2003 Sep;25(17):1415-9. doi: 10.1023/a:1025047410699.
Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When glucose was kept at 2.5 g l(-1), cell growth and PHB synthesis were limited due to the shortage of carbon source but a higher PHB content occurred in the cell-growth stage. This shows that a low glucose concentration is favorable for PHB accumulation in R. eutropha. PHB obtained with glucose at 9 g l(-1) is 1.6 times that obtained with 40 g l(-1). When glucose was in the range of 9 to 40 g l(-1), PHB concentration and productivity decreased significantly with the increase of glucose concentration. The highest PHB productivity was obtained with glucose at 9 g l(-1).
通过在不同葡萄糖浓度下对真养产碱杆菌进行磷酸盐限制的补料分批培养来生产聚（3-羟基丁酸酯）（PHB）。当葡萄糖浓度保持在2.5 g l⁻¹时，由于碳源短缺，细胞生长和PHB合成受到限制，但在细胞生长阶段细胞内的PHB含量较高。这表明低葡萄糖浓度有利于真养产碱杆菌中PHB的积累。葡萄糖浓度为9 g l⁻¹时获得的PHB是40 g l⁻¹时的1.6倍。当葡萄糖浓度在9至40 g l⁻¹范围内时，PHB浓度和生产率随着葡萄糖浓度的增加而显著降低。葡萄糖浓度为9 g l⁻¹时获得了最高的PHB生产率。
