利用贪铜菌以大豆油为碳源生产聚羟基丁酸（PHB），脉冲式补料以获得高产和高生产力。
Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator.
机构信息
Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil.
出版信息
Biotechnol Lett. 2012 Jun;34(6):1003-7. doi: 10.1007/s10529-012-0863-1.
Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l(-1)) was achieved using soybean oil at 40 g l(-1) and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l(-1) h(-1), and the calculated Y(p/s) value was 0.85 g g(-1). Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe.
利用台式生物反应器研究了贪铜菌从大豆油中合成聚（3-羟基丁酸酯）（PHB）。当大豆油浓度为 40 g/L 并添加相同浓度的脉冲时，可获得最高的细胞浓度（83 g/L）。PHB 含量为 81%（w/w），PHB 生产力为 2.5 g/L/h，计算得出的 Y(p/s) 值为 0.85 g/g。由于 P 以及可能还有 Cu、Ca 和 Fe 的耗尽，导致生长受限和 PHB 生物合成的开始。
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