在甲基营养型酵母毕赤酵母中表达的重组雪花莲凝集素（GNA）的大规模生产与纯化。
Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris.
作者信息
Baumgartner Philippe, Harper Karen, Raemaekers Romaan J M, Durieux Alain, Gatehouse Angharad M R, Davies Howard V, Taylor Mark A
机构信息
Quality, Health & Nutrition Group, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland.
出版信息
Biotechnol Lett. 2003 Aug;25(15):1281-5. doi: 10.1023/a:1025007901322.
The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l(-1) at the 200 1 scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.
来自雪花莲（Galanthus nivalis）的凝集素（GNA）编码基因在甲基营养型酵母毕赤酵母（Pichia pastoris）中表达并分泌。筛选出了毕赤酵母转化体，并开发了一种生产和纯化克级量重组GNA的方法。在200升规模下，GNA的分泌量约为80毫克/升，并使用疏水相互作用色谱法将其纯化至95%的纯度。重组蛋白在结构和生物活性方面与植物中合成的蛋白相似。
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