从天然来源中纯化与GNA相关的凝集素。
Purification of GNA-Related Lectins from Natural Sources.
作者信息
Van Damme Els J M
机构信息
Laboratory of Glycobiology and Biochemistry, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
出版信息
Methods Mol Biol. 2020;2132:413-419. doi: 10.1007/978-1-0716-0430-4_40.
PMID:32306348
Abstract
The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.
摘要
雪花莲凝集素，缩写为GNA，是一大类甘露糖结合凝集素的模型蛋白。在此，我们描述了从干燥鳞茎开始纯化GNA的方法。通过离子交换色谱法和甘露糖-琼脂糖亲和色谱法相结合，可以获得高纯度的GNA制剂。
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