Zhang Qin, Liao Zhen-jiang, Wang Xin, Liu Jian, Jin Zai-ming, Xu Li-jv
Department of Burns, Rui Jin Hospital, The Second Medical University of Shanghai, PR China.
Zhonghua Shao Shang Za Zhi. 2003 Aug;19(4):216-8.
To investigate the changes in the systemic inflammatory response and T cell induced immunity after systemic administration of recombinant human growth hormone (rhGH) during early postburn stage.
Forty Sprague-Dawley (SD) rats were randomly divided into three groups for the study. The rats in control group (C, n = 6) were only used for the determination of plasma levels of the cytokines, such as TNFalpha, IL-2, IL-6 and CD4(+) and CD8(+) cells. The SD rats in burn with rhGH treatment group (BT, n = 18) and burn without treatment group (B, n = 18) were inflicted with III degree scalding injury on the back. rhGH was injected subcutaneously on the abdomen of the rats in a dose of 6 IU/kg for 10 days in BT group. The blood samples were harvested from the rats in the two groups for the evaluation of the above indices.
The plasma levels of TNFalpha, IL-2, IL-6 and CD4(+) and CD8(+) cells were increased on the 3(rd) postburn day (PBD) and decreased on the 6(th) PBD in B group, while the CD4(+) and CD8(+) cells were increased significantly and the plasma levels of TNFalpha, IL-2, IL-6 decreased obviously on the 3(rd) PBD in BT group. And the plasma levels of IL-2 and IL-6 in BT group on the 6(th) PBD showed no difference from those in C group. But the plasma TNFalpha level in BT group was evidently higher than that in B and C group on the 6(th) PBD. Furthermore, the plasma levels of TNFalpha, IL-2 and IL-6 in BT group were still increased gradually on the 10(th) PBD, while the IL-2 and IL-6 levels were decreased obviously in B group, but the TNFalpha level was increased.
Systemic administration of rhGH during different states of stress exerted different effects on T cell induced immunity and systemic inflammatory response.
探讨烧伤早期全身应用重组人生长激素（rhGH）后全身炎症反应及T细胞介导免疫的变化。
40只Sprague-Dawley（SD）大鼠随机分为三组进行研究。对照组（C组，n = 6）大鼠仅用于测定血浆细胞因子水平，如肿瘤坏死因子α（TNFα）、白细胞介素-2（IL-2）、白细胞介素-6（IL-6）以及CD4(+)和CD8(+)细胞。烧伤并rhGH治疗组（BT组，n = 18）和烧伤未治疗组（B组，n = 18）的SD大鼠背部均造成Ⅲ度烫伤。BT组大鼠于腹部皮下注射rhGH，剂量为6 IU/kg，共10天。采集两组大鼠血样以评估上述指标。
B组大鼠烧伤后第3天（PBD）血浆TNFα、IL-2、IL-6水平及CD4(+)和CD8(+)细胞数量升高，第6天PBD降低；而BT组大鼠烧伤后第3天PBD时CD4(+)和CD8(+)细胞数量显著增加，血浆TNFα、IL-2、IL-6水平明显降低。BT组大鼠烧伤后第6天PBD时IL-2和IL-6血浆水平与C组无差异。但BT组大鼠烧伤后第6天PBD时血浆TNFα水平明显高于B组和C组。此外，BT组大鼠烧伤后第10天PBD时血浆TNFα、IL-2和IL-6水平仍逐渐升高，而B组IL-2和IL-6水平明显降低，但TNFα水平升高。
在不同应激状态下全身应用rhGH对T细胞介导免疫和全身炎症反应产生不同影响。
