DOU Yi, ZHANG Qin, ZHANG Xiong, DONG Jiao-yun, TANG Jia-jun, LIAO Zhen-jiang
Department of Burns and Plastic Surgery, Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, China.
Zhonghua Shao Shang Za Zhi. 2009 Aug;25(4):275-80.
To investigate the effect of Thymosin and growth hormone(GH) on inflammatory response in burn rats or burn rats with sepsis.
Sixty-four SD rats were randomly divided into normal control group (NC, without treatment), sepsis group (S, with injection of LPS), sepsis + Thymosin group (ST, with successive injection of Thymosin and LPS), sepsis + GH group [SGH, with successive injection of recombinant human GH (rhGH) and LPS], burn group, burn + sepsis group (BS, with injection of LPS after burn), burn + sepsis + Thymosin group (BST, with successive injection of Thymosin and LPS after burn), burn + sepsis + GH (BSGH, with successive injection of rhGH and LPS after burn), with 8 rats in each group. Specimens of spleen tissues were harvested to determine HLA-DR in lymphocyte and evaluate inflammatory cell infiltration (score). Specimens of peripheral blood were collected to determine Toll-like receptor 4 (TLR4) level in monocyte and serum level of TNF-alpha, IL-4, IL-6, IL-10.
Compared with those in NC group, serum level of IL-10 in S group decreased obviously, while other indices increased obviously (P < 0.01). The levels of HLA-DR and TLR4 and serum level of TNF-alpha were similar between SGH and ST groups (P > 0.05). Compared with those in SGH group [(2.87 +/- 0.04) score, and IL-6 (0.0083 +/- 0.0018) microg/mg, IL-4 (0.0102 +/- 0.0021) microg/mg, IL-10 (0.0310 +/- 0.0027) microg/mg, respectively], degree of inflammatory cell infiltration (1.50 +/- 0.76) score and serum levels of IL-6, IL-4, IL-10 of rats in ST group decreased obviously (0.0064 +/- 0.0012, 0.0058 +/- 0.0024, 0.0230 +/- 0.0021 microg/mg, respectively, P < 0.01). The levels of HLA-DR, TLR4 and inflammatory cell infiltration degree of spleen in B group were respectively higher than those in NC group and lower than those in BS group. Compared with those in NC group, serum levels of TNF-alpha, IL-6 in B group increased significantly, while IL-4, IL-10 showed an opposite tendency. There was no obvious difference between BST and BSGH groups in serum levels of HLA-DR and IL-6 (P > 0.05). Compared with those in BST group, inflammatory cell infiltration degree in spleen and the levels of TLR, TNF-alpha obviously decreased (P < 0.01), while IL-4 and IL-10 levels increased in BSGH group (P < 0.01).
Inhibitive effects between Thymosin and GH on extensive inflammatory reaction were similar with or without trauma, and GH has better effect as compared with Thymosin when with trauma.
探讨胸腺肽与生长激素（GH）对烧伤大鼠或合并脓毒症烧伤大鼠炎症反应的影响。
将64只SD大鼠随机分为正常对照组（NC，未处理）、脓毒症组（S，注射脂多糖）、脓毒症+胸腺肽组（ST，先后注射胸腺肽和脂多糖）、脓毒症+GH组[SGH，先后注射重组人生长激素（rhGH）和脂多糖]、烧伤组、烧伤+脓毒症组（BS，烧伤后注射脂多糖）、烧伤+脓毒症+胸腺肽组（BST，烧伤后先后注射胸腺肽和脂多糖）、烧伤+脓毒症+GH组（BSGH，烧伤后先后注射rhGH和脂多糖），每组8只。采集脾脏组织标本，检测淋巴细胞表面人类白细胞抗原-DR（HLA-DR）并评估炎症细胞浸润情况（评分）。采集外周血标本，检测单核细胞表面Toll样受体4（TLR4）水平及血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）水平。
与NC组相比，S组血清IL-10水平明显降低，其他指标明显升高（P<0.01）。SGH组与ST组的HLA-DR、TLR4水平及血清TNF-α水平相似（P>0.05）。与SGH组[炎症细胞浸润程度评分为（2.87±0.04）分，IL-6为（0.0083±0.0018）μg/mg，IL-4为（0.0102±0.0021）μg/mg，IL-10为（0.0310±0.0027）μg/mg]相比，ST组大鼠炎症细胞浸润程度评分（1.50±0.76）分及血清IL-6、IL-4、IL-10水平明显降低（分别为0.0064±0.0012、0.0058±0.0024、0.0230±0.0021μg/mg，P<0.01）。B组脾脏HLA-DR、TLR4水平及炎症细胞浸润程度分别高于NC组且低于BS组。与NC组相比，B组血清TNF-α、IL-6水平显著升高，IL-4、IL-10呈相反趋势。BST组与BSGH组血清HLA-DR及IL-6水平差异无统计学意义（P>0.05）。与BST组相比，BSGH组脾脏炎症细胞浸润程度及TLR、TNF-α水平明显降低（P<0.01），而IL-4及IL-10水平升高（P<0.01）。
胸腺肽与GH对广泛炎症反应的抑制作用在有无创伤情况下相似，且在有创伤时GH的作用优于胸腺肽。
