快速、同时且灵敏地检测葡萄球菌。
Fast, simultaneous, and sensitive detection of staphylococci.
作者信息
Stuhlmeier R, Stuhlmeier K M
机构信息
Ludwig Boltzmann Institute for Rheumatology and Balneology, Kurbadstrasse 10, 1100 Vienna, Austria.
出版信息
J Clin Pathol. 2003 Oct;56(10):782-5. doi: 10.1136/jcp.56.10.782.
AIMS
Bacterial infections are common and are involved in many forms of disease, ranging from arthritis to food poisoning. Of much concern are nosocomial infections, especially the increasing resistance of bacteria to methicillin. A prerequisite for the successful treatment of bacterial infections is a specific and sensitive method of detecting microorganisms.
METHODS
Some methods to detect bacteria are time consuming, whereas others are faster but lack specificity and/or sensitivity. This article describes an optimised polymerase chain reaction (PCR) method that enables the simultaneous detection of different bacteria. A prerequisite for sensitive PCR is a method to isolate and recover extremely small amounts of bacterial DNA. This study used a new method to isolate DNA and compared the results to an established method.
RESULTS
The method could detect fewer than 10 Staphylococcus aureus, methicillin resistant S aureus, Staphylococcus epidermitis, and other bacteria and it took less than two hours to perform.
CONCLUSION
The rapid DNA isolation method used in conjunction with the optimised PCR makes it possible to confirm the presence or absence of extremely small numbers of bacteria. Using real time PCR would shorten the procedure even further. This method might therefore contribute to more timely and specific interventions.
目的
细菌感染很常见，涉及多种疾病形式，从关节炎到食物中毒。医院感染备受关注，尤其是细菌对甲氧西林的耐药性不断增加。成功治疗细菌感染的一个先决条件是一种特异且灵敏的微生物检测方法。
方法
一些检测细菌的方法耗时，而其他方法虽更快但缺乏特异性和/或灵敏性。本文描述了一种优化的聚合酶链反应（PCR）方法，可同时检测不同细菌。灵敏PCR的一个先决条件是一种分离和回收极少量细菌DNA的方法。本研究使用一种新的DNA分离方法，并将结果与一种既定方法进行比较。
结果
该方法能检测出少于10个金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、表皮葡萄球菌及其他细菌，且检测过程耗时不到两小时。
结论
与优化的PCR结合使用的快速DNA分离方法，能够确认极少量细菌的存在与否。使用实时PCR将进一步缩短检测过程。因此，该方法可能有助于更及时和特异的干预措施。
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