[溴化乙锭与单链DNA的连接]
[Connection of ethidium bromide with single-stranded DNA].
作者信息
Vardevanian P O, Antonian A P, Parsadanian M A, Lavtian A G, Karapetian A T
机构信息
Biological Department, Yerevan State University, ul. A. Manukyana 1, Yerevan, 375025 Armenia.
出版信息
Biofizika. 2003 Jul-Aug;48(4):644-7.
The interaction of ethidium bromide with single-stranded synthetic and natural polynucleotides at high temperatures (t = 70 degrees C) and low pH values (pH 3.0) was studied. The isotherms of adsorption of ethidium bromide on single-stranded DNA were obtained. Two modes of binding of single-stranded DNA, strong and weak, were revealed. The values of the corresponding constants of interaction of this ligand and the number of bases per one binding site were determined.
研究了溴化乙锭在高温（t = 70℃）和低pH值（pH 3.0）条件下与单链合成多核苷酸和天然多核苷酸的相互作用。获得了溴化乙锭在单链DNA上的吸附等温线。揭示了单链DNA的两种结合模式，即强结合和弱结合。测定了该配体相应的相互作用常数的值以及每个结合位点的碱基数。
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