Cong W M, Wu M C, Zhang X Z
Department of Pathology, Changhai Hospital, Shanghai.
Chin Med J (Engl). 1992 Jul;105(7):535-8.
DNA stemlines of hepatocellular carcinoma (HCC) model of adult Wistar rats established by diethylnitrosamine were quantitatively measured by flow cytometry. The cancer-inducing course was divided into four stages, eg, precirrhosis stage, cirrhosis stage, early cancer stage and cancer progression stage. The advantageous DNA stemline of hepatocytes of normal adult Wistar rats was tetraploid (4C). It was from the cirrhosis stage that atavistic proliferation of hepatocytes with diploid (2C) DNA stemline started and replaced 4C hepatocytes, resulting in a new advantageous population. The features of early cancer stage were formation of HCC with 2C DNA stemline, whereas during the cancer progression stage, HCC cells with aneuploid (AN) DNA stemline presented the advantageous growth. The study clearly shows the change pattern of DNA stemline during the course of hepatocarcinogenesis from 4C-2C-AN, which is believed to be the biokinetic mechanism of the development and progression of HCC.
采用流式细胞术对用二乙基亚硝胺建立的成年Wistar大鼠肝细胞癌（HCC）模型的DNA干系进行定量测定。致癌过程分为四个阶段，即肝硬化前期、肝硬化期、早期癌阶段和癌进展阶段。正常成年Wistar大鼠肝细胞的优势DNA干系为四倍体（4C）。从肝硬化期开始，具有二倍体（2C）DNA干系的肝细胞开始返祖增殖并取代4C肝细胞，从而产生一个新的优势群体。早期癌阶段的特征是形成具有2C DNA干系的HCC，而在癌进展阶段，具有非整倍体（AN）DNA干系的HCC细胞呈现优势生长。该研究清楚地显示了肝癌发生过程中DNA干系从4C-2C-AN的变化模式，这被认为是HCC发生发展的生物动力学机制。
