Onodera K, Ebata H, Kasai S, Mito M
Sapporo Hokuyu Hospital, Japan.
Nihon Geka Gakkai Zasshi. 1992 Sep;93(9):932-6.
It is important to distinguish a precancerous lesion and hepatocellular carcinoma (HCC) with diploidy or aneuploidy nuclear DNA pattern, not only in clinical cases but also in experimental carcinogenesis models. Using liver perfusion technique, we detected early HCC from persistent hyperplastic nodules (HN) which were induced in Wistar rats by intermittent 5-6 months administration of 2-acetylaminofluorene. This investigation was undertaken to assess both promotive and progressive effects of liver regeneration following partial hepatectomy (PH). Results are as follows: 1) Isolated hepatocytes of precancerous HN, which were transplanted into the spleen, didn't develop to HCC by 2 months after 70% PH of host liver. 2) Diced tissues of HCC, which were transplanted into the liver via portal vein, grew many metastasis in 10/10 by 7 weeks after PH, while 5/19 in control. 3) Nuclear DNA patterns of early HN-late HCC in rat liver were diploidy at the rate of more than 90% each. But it changed to aneuploidy, when inoculation of HCC for one month was repeated 7 times in the spleen.
区分癌前病变和具有二倍体或非整倍体核DNA模式的肝细胞癌（HCC）很重要，这不仅在临床病例中，而且在实验性致癌模型中都是如此。我们采用肝脏灌注技术，从经间歇性给予2-乙酰氨基芴5至6个月诱导形成的Wistar大鼠持续性增生性结节（HN）中检测早期HCC。本研究旨在评估部分肝切除（PH）后肝脏再生的促进和进展作用。结果如下：1）将癌前HN的分离肝细胞移植到脾脏中，在宿主肝脏70% PH后2个月内未发展为HCC。2）通过门静脉将HCC的切碎组织移植到肝脏中，在PH后7周时，10只中有10只出现多处转移，而对照组为19只中有5只。3）大鼠肝脏中早期HN至晚期HCC的核DNA模式各有超过90%的比例为二倍体。但当在脾脏中重复接种HCC 1个月7次时，其转变为非整倍体。
