Wang C F, Ju M C, Shi Z R, Sun W R, Jin M L, Niu Y J, Liu J G, Ge F Y
Department of Ophthalmology, Affiliated Hospital Qingdao Medical College.
Chin Med J (Engl). 1992 Jul;105(7):572-6.
Since March 1986, the authors have studied and simplified the corneal cryopreservation technique using a self-made QL Bio-freezer. On the basis of successful animal experiments, penetrating keratoplasties with human cadaver corneas cryopreserved 9 to 571 days (averaging 131 days) were performed in 60 patients (61 eyes). The patients were followed up for 1.5 to 50 months. 41 of the corneal grafts remained clear, 9 became translucent and 11 cloudy. A total of 46 eyes had their visual acuity improved significantly. The results show that clinical use of corneal cryopreservation by the simplified two-step method is noteworthy.
自1986年3月以来，作者使用自制的QL生物冷冻器对角膜冷冻保存技术进行了研究并加以简化。在动物实验成功的基础上，对60例（61只眼）患者实施了穿透性角膜移植术，所用角膜为冷冻保存9至571天（平均131天）的人尸体角膜。对患者进行了1.5至50个月的随访。41例角膜移植片保持透明，9例变为半透明，11例混浊。共有46只眼的视力显著提高。结果表明，简化的两步法角膜冷冻保存技术在临床上的应用值得关注。
