Usson Y, Humbert C
Laboratoire TIM3, USR 00690B C.N.R.S., Université Joseph Fourier, Grenoble, France.
Cytometry. 1992;13(6):595-602. doi: 10.1002/cyto.990130607.
The observation of BrdUrd staining in the nuclei of cells from exponentially growing populations reveals different typical replicating patterns. We propose a methodological approach to order and characterize BrdUrd intranuclear distributions. First, visual ordering of the patterns is assessed using a spectral analysis coupled to a k-nearest neighbors clustering technique. Subsequently, nine topographical features are introduced to characterize the spatial distribution of BrdUrd-tagged fluorescence in the nuclei of proliferating cells. These topographical features are based on a structural approach. The localization of fluorescence spots is expressed in terms of the normalized distance from the nuclear border and its standard deviation. These topographical features are simple to calculate and easy to relate to visual experience.
对指数增长群体的细胞进行BrdUrd染色观察，发现细胞核内存在不同的典型复制模式。我们提出了一种方法来对BrdUrd核内分布进行排序和表征。首先，使用光谱分析结合k近邻聚类技术对模式进行视觉排序。随后，引入九个地形特征来表征增殖细胞核内BrdUrd标记荧光的空间分布。这些地形特征基于一种结构方法。荧光点的定位通过距核边界的归一化距离及其标准差来表示。这些地形特征计算简单，且易于与视觉经验相关联。
