Humbert C, Usson Y
Laboratoire TIM3, USR CNRS 00690B, Université Joseph Fourier, Grenoble, France.
Cytometry. 1992;13(6):603-14. doi: 10.1002/cyto.990130608.
This paper describes the relationship between the BrdUrd replicating pattern of a cell and its localization within the S phase by means of topographical features and DNA content measurement. The present study follows an objective ranking of the BrdUrd patterns obtained from a spectral analysis of the BrdUrd images. The pattern ranking was consistent with the DNA content increase throughout the S phase. Five texture groups were arbitrarily set up for the purpose of multivariate analysis. Nine topographical parameters were computed for each BrdUrd-labelled nucleus. The descriptive quality of these parameters was assessed by means of factorial discriminant analysis. These parameters made it possible to characterize objectively the known pattern distributions of replication sites qualitatively described in the literature.
本文通过地形特征和DNA含量测量描述了细胞的BrdUrd复制模式与其在S期内定位之间的关系。本研究对从BrdUrd图像光谱分析中获得的BrdUrd模式进行了客观排序。模式排序与整个S期的DNA含量增加一致。为了进行多变量分析，任意设置了五个纹理组。为每个BrdUrd标记的细胞核计算了九个地形参数。通过因子判别分析评估了这些参数的描述质量。这些参数使得能够客观地表征文献中定性描述的复制位点的已知模式分布。
