van den Bos C, van Gils F C, Bartstra R W, Wagemaker G
Department of Radiobiology, Erasmus University, Rotterdam, The Netherlands.
Cytometry. 1992;13(6):659-62. doi: 10.1002/cyto.990130616.
A rapid and reliable method for longitudinal studies on the degree of red cell chimerism following bone marrow transplantation of alpha-thalassemic recipient mice is presented. Blood obtained by tail clipping from transplanted mice was analyzed by measuring forward light scatter (FLS) distribution of red cells using a flow cytometer. Amplification and threshold of FLS were specifically adjusted. For flow cytometric analysis, the red cells needed to be suspended in hypotonic saline (103 mmol/l NaCl). Osmotic fragility testing showed that lysis of erythrocytes did not significantly influence the measurements. Flow cytometric measurement allowed for a rapid determination of the degree of red cell chimerism.
本文介绍了一种用于纵向研究α地中海贫血受体小鼠骨髓移植后红细胞嵌合度的快速可靠方法。通过剪尾从移植小鼠获取血液，使用流式细胞仪测量红细胞的前向光散射（FLS）分布来进行分析。FLS的放大倍数和阈值经过专门调整。对于流式细胞术分析，红细胞需悬浮于低渗盐溶液（103 mmol/l NaCl）中。渗透脆性测试表明红细胞裂解对测量结果无显著影响。流式细胞术测量能够快速确定红细胞嵌合度。
