Rink Anette, Eyer Katie, Roelofs Benjamin, Priest Kimberly J, Sharkey-Brockmeier Kathleen J, Lekhong Sudkamon, Karajusuf Elif K, Bang Jason, Yerle Martine, Milan Denis, Liu Wan-Sheng, Beattie Craig W
Department of Animal Biotechnology, College of Agriculture, Biotechnology and Natural Resources, University of Nevada, Reno, Nevada 89557, USA.
Mamm Genome. 2006 Aug;17(8):878-85. doi: 10.1007/s00335-005-0121-0. Epub 2006 Aug 4.
The IMpRH(7000-rad) radiation hybrid panel was used to map 2035 expressed sequence tags (ESTs) at a minimum LOD score of 4.0. A total of 134 linkage groups covers 57,192 cR or 78% of the predicted size of the porcine and 71% of the human genome, respectively. Approximately 81% (1649) of the porcine ESTs were annotated against the NCBI nonredundant database; 1422 mapped in silico to a location in build 35.1 of the human genome sequence (HGS) and 1185 to a gene and location in build 35.1 HGS. The map revealed 40 major breaks in synteny (1.00e (-25 )and lower) with the human genome, 37 of which fall within a single chromosome. At this improved level of resolution and coverage, porcine chromosomes (SSC) 2, 5, 6, 7, 12, and 14 remain "gene-rich" and homologous to human chromosomes (HSA) 17, 19, and 22, while SSC 1, 8, 11, and X have been confirmed to correspond to the "gene-deserts" on HSA 18, 4, 13, and X.
使用IMpRH(7000拉德)辐射杂种细胞系图谱，以最低LOD分数4.0对2035个表达序列标签(ESTs)进行定位。总共134个连锁群分别覆盖了猪基因组预测大小的57,192厘雷或78%以及人类基因组预测大小的71%。大约81%(1649个)猪ESTs与NCBI非冗余数据库进行了比对注释；其中1422个在计算机上定位到人类基因组序列(HGS)构建版35.1中的一个位置，1185个定位到构建版35.1 HGS中的一个基因及位置。该图谱揭示了与人类基因组在同线性上有40个主要断点(1.00e(-25)及更低)，其中37个断点位于单条染色体上。在这种更高分辨率和覆盖率水平下，猪染色体(SSC)2、5、6、7、12和14仍然“基因丰富”，并与人类染色体(HSA)17、19和22同源，而SSC 1、8、11和X已被证实分别对应于HSA 18、4、13和X上的“基因荒漠”。
