Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland.
Mech Dev. 2002 Dec;119 Suppl 1:S107-10. doi: 10.1016/s0925-4773(03)00101-1.
We describe the expression of three Runt-related RUNX genes (previously termed AML, Cbfa, or Pebp2alpha) Runx1 and Runx3 during the development of teeth and other craniofacial tissues and compare them to Runx2 expression reported earlier. All three genes were expressed in mesenchymal condensates. Runx1 was expressed in several cartilage primordia earlier than Runx3, and Runx2 was intense in all mesenchymal condensations of bones and teeth. Only Runx1 was expressed in epithelia, and in tooth germs transcripts were detected in outer dental epithelium. Runx1 was also intensely expressed in the midline epithelium of palatal shelves. In early tooth morphogenesis Runx3 was coexpressed with Runx2 in a thin layer of mesenchymal cells underlying dental epithelium. Unlike Runx2, Runx3 was expressed in odontoblasts. However, Runx3 mutant mice did not show obvious tooth phenotype or deviations of Runx1 and Runx2 expression patterns in the tooth.
我们描述了三个 Runt 相关的 RUNX 基因（以前称为 AML、Cbfa 或 Pebp2alpha）Runx1 和 Runx3 在牙齿和其他颅面组织发育过程中的表达，并将其与之前报道的 Runx2 表达进行了比较。这三个基因都在间充质凝聚物中表达。Runx1 的表达比 Runx3 早于几个软骨原基，而 Runx2 在骨骼和牙齿的所有间充质凝聚物中都强烈表达。只有 Runx1 在上皮中表达，在外胚层牙胚中检测到转录本。Runx1 在腭突的中线上皮中也强烈表达。在早期牙齿形态发生过程中，Runx3 与 Runx2 在牙上皮下的一层薄的间充质细胞中共表达。与 Runx2 不同，Runx3 在成牙本质细胞中表达。然而，Runx3 突变小鼠并没有表现出明显的牙齿表型，也没有表现出 Runx1 和 Runx2 表达模式在牙齿中的偏差。
