Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Mech Dev. 2002 Dec;119 Suppl 1:S197-202. doi: 10.1016/s0925-4773(03)00116-3.
In this study, we describe the isolation and characterization of Foxp4, a new member of the Foxp subfamily of winged-helix transcription factors. The full-length mouse Foxp4 cDNA encodes a 685-amino-acid protein that is similar to Foxp1 and Foxp2. Foxp4 gene expression is observed primarily in pulmonary, neural, and gut tissues during embryonic development. To compare the protein expression patterns of Foxp4 to Foxp1 and Foxp2, specific polyclonal antisera to each of these proteins was used in immunohistochemical analysis of mouse embryonic tissues. All three proteins are expressed in lung epithelium with Foxp1 and Foxp4 expressed in both proximal and distal airway epithelium while Foxp2 is expressed primarily in distal epithelium. Foxp1 protein expression is also observed in the mesenchyme and vascular endothelial cells of the lung. At embryonic day 12.5, Foxp1 and Foxp2 are expressed in both the mucosal and epithelial layers of the intestine. However, Foxp2 is expressed only in the outer mucosal layer of the intestine and stomach later in development. Finally, Foxp4 is expressed exclusively in the epithelial cells of the developing intestine, where, in late development, it is expressed in a gradient along the longitudinal axis of the villi.
在这项研究中，我们描述了 Foxp4 的分离和特性，Foxp4 是新的具有翼状螺旋转录因子结构域的 Foxp 亚家族的成员。全长的小鼠 Foxp4 cDNA 编码一个 685 个氨基酸的蛋白质，它与 Foxp1 和 Foxp2 相似。Foxp4 基因的表达在胚胎发育过程中主要观察到在肺、神经和肠道组织中。为了比较 Foxp4 蛋白与 Foxp1 和 Foxp2 的表达模式，我们使用特异性的多克隆抗血清对这些蛋白在小鼠胚胎组织中的免疫组织化学分析。这三种蛋白都在肺上皮中表达，Foxp1 和 Foxp4 在近端和远端气道上皮中都有表达，而 Foxp2 主要在远端上皮中表达。Foxp1 蛋白的表达也在肺的间质和血管内皮细胞中观察到。在胚胎第 12.5 天，Foxp1 和 Foxp2 在肠的黏膜和上皮层中都有表达。然而，Foxp2 仅在肠和胃的外层黏膜层中表达，在发育后期。最后，Foxp4 仅在发育中的肠上皮细胞中表达，在后期发育中，它沿着绒毛的纵轴呈梯度表达。
